il 10 specific antibodies  (Boster Bio)

Journal: Bioactive Materials

Article Title: Glucose/ROS-responsive and redox-gated adaptive hydrogel dressing for accelerating diabetic wound repair via synergistic cGAS/STING pathway inhibition and oxidative stress alleviation

doi: 10.1016/j.bioactmat.2026.03.025

Figure Lengend Snippet: Angiogenesis and collagen deposition in diabetic wound tissues following HPSL@SG hydrogel treatment. (A) Dihydroethidium (DHE) immunofluorescence staining and (B) semi-quantitative analysis of wound tissues from each treatment group on day 7, scale bar = 100 μm. Immunofluorescence staining of (C) MMP-9, IL-6, and IL-10, and (D) CD31, VEGF-A, and collagen I in wound tissue sections from each treatment group on day 7, scale bar = 100 μm. (E-J) Mean relative fluorescence intensity of each indicator in wound tissue sections from each treatment group on day 7, scale bar = 100 μm. All data are shown as mean ± SEM (n = 6).

Article Snippet: IL-6 and IL-10-specific antibodies were purchased from Bosterbio (Wuhan, China).

Techniques: Immunofluorescence, Staining, Fluorescence

Journal: PLoS ONE

Article Title: In Vivo Induction of Tr1 Cells via Mucosal Dendritic Cells and AHR Signaling

doi: 10.1371/journal.pone.0023618

Figure Lengend Snippet: A. Tiger mice were nasally treated with IC (clear bars) or anti-CD3 (filled bars) and 72 hrs after the last nasal dose GFP(IL-10) expression by CD4+ T cells in CLN was examined by flow cytometry. This experiment was repeated 4 times with same results. B and C. CD4+CD25-GFP-, CD4+CD25+GFP- or CD4+CD25-GFP+ T cells were sorted from CLN of Tiger mice nasally treated with IC (clear bar) or anti-CD3 (filled bar). Sorted T cells were stimulated in vitro with plate bound anti-CD3 and anti-CD28 antibodies (1 µg/ml each) and IL-10 ( B ) and ( C ) IFN-γ were detected in the supernatants by ELISA. Error bars represent standard deviations and P values were calculated by t-test. D. The percentage of CD4+CD25-LAP+ T cells that express IL-10 following nasal anti-CD3 was assessed by intracellular staining. Each symbol represents an individual mouse. E . FACS-sorted Tr1 cells (CD4+CD25-GFP(IL-10)+, clear bar) from CLN of nasal anti-CD3 treated Tiger mice or IL-27 in vitro differentiated Tr1 cells (filled bar) were used in a standard suppression assay with naïve CD4+CD25-GFP- responder T cells at various ratios. To test the role of IL-10 in in vitro suppression, IC or anti-IL-10 (50 µg/ml) neutralizing antibodies were added to co-cultures at 1∶1 ratio.

Article Snippet: Fluorescent anti-mouse antibodies used in flow cytometry were CD4-specific (H129.19), CD25-specific (PC61) and IL-10 (JES5-16E3) (BD Biosciences).

Techniques: Expressing, Flow Cytometry, In Vitro, Enzyme-linked Immunosorbent Assay, Staining, Suppression Assay

Journal: PLoS ONE

Article Title: In Vivo Induction of Tr1 Cells via Mucosal Dendritic Cells and AHR Signaling

doi: 10.1371/journal.pone.0023618

Figure Lengend Snippet: A. Naive CD4+ T cells (CD4+CD25-GFP(IL-10)-), nTregs (CD4+CD25+GFP(foxp3)+) sorted from Foxp3-GFP knock-in mice, ex vivo Tr1 cells (CD4+CD25-GFP(IL10)+) sorted from CLN of nasal anti-CD3 treated Tiger mice and in vitro differentiated Tr1 using plate bound anti-CD3 and anti-CD28 plus 50 ng/ml IL-27 were used in quantitative RTPCR reactions. Expressions of IL-10, IFN-γ and foxp3 mRNA were normalized to expression of β-actin. B. AHR, C. cMAF, D. IL-21 and E. IL-21R mRNA expression by CD4+GFP(IL-10)- T cells or CD4+GFP(IL-10)+ Tr1 cells. These experiments were repeated 3 times with same results.

Article Snippet: Fluorescent anti-mouse antibodies used in flow cytometry were CD4-specific (H129.19), CD25-specific (PC61) and IL-10 (JES5-16E3) (BD Biosciences).

Techniques: Knock-In, Ex Vivo, In Vitro, Reverse Transcription Polymerase Chain Reaction, Expressing

Journal: PLoS ONE

Article Title: In Vivo Induction of Tr1 Cells via Mucosal Dendritic Cells and AHR Signaling

doi: 10.1371/journal.pone.0023618

Figure Lengend Snippet: A. Tiger or Ahr d /tiger mice were nasally treated with IC or anti-CD3 and 72 hrs after the last nasal dose GFP(IL-10) expression by CD4+ T cells in CLN was examined by flow cytometry. Each symbol represents an individual mouse. B. Tiger mice were nasally treated with IC or anti-CD3 alone or together with recombinant mouse IL-21 (4 µg/day). C. WT or IL-21R−/− mice were nasally treated with IC or anti-CD3.

Article Snippet: Fluorescent anti-mouse antibodies used in flow cytometry were CD4-specific (H129.19), CD25-specific (PC61) and IL-10 (JES5-16E3) (BD Biosciences).

Techniques: Expressing, Flow Cytometry, Recombinant

Journal: PLoS ONE

Article Title: In Vivo Induction of Tr1 Cells via Mucosal Dendritic Cells and AHR Signaling

doi: 10.1371/journal.pone.0023618

Figure Lengend Snippet: Nasal anti-CD3 triggers T cell activation via TCR/CD3 complex. T cell activation in the presence of IL-27 secreted by local DCs in the CLN leads to activation of AHR and cMAF, which cooperate to transactivate the il10 and il21 promoters and promote IL-10 and IL-21 production. IL-21 then acts as a Tr1 growth factor in an autocrine fashion.

Article Snippet: Fluorescent anti-mouse antibodies used in flow cytometry were CD4-specific (H129.19), CD25-specific (PC61) and IL-10 (JES5-16E3) (BD Biosciences).

Techniques: Activation Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: New Insights Into Blue Light Phototherapy in Experimental Trypanosoma cruzi Infection

doi: 10.3389/fcimb.2021.673070

Figure Lengend Snippet: Level of plasma cytokines in C57BL/6 mice under blue light phototherapy. T. cruzi -infected animals were exposed to conventional and blue light for 9 days, 12 h per day. The plasma was collected to evaluate the cytokines TNF (A) , IL-6 (B) , CCL2 (C) and IL-10 (D) production. p<0.05 means differences between infected groups under blue light and conventional light.

Article Snippet: The pH was adjusted to 7.4, and the samples were added to wells of a 96-well plate previously coated with monoclonal antibodies specific for TNF, IL-6, CCL2, and IL-10 (PeproTech, Cranbury, NJ, USA) according to the manufacturer’s protocols.

Techniques: Infection